Images
- HON Logo
- RSS Feeds
- Fig. 1
- Fig. 2
- Fig.3
- Fig.4
- Fig. 1
- Fig. 2
- Fig. 1
- Fig.3
- Fig. 1
- fig2
- Fig.3
- Team D. Lévy. Functional and structural analysis of membrane proteins
- Fig. 2
- fig 3
- Fig.4
- Fig. 2
- Fig. 2
- Fig.3
- Fig. 1
- Fig. 2
- Fig.3
- fig.4
- fig1
- fig.2
- Fig.3
- fig.4
- Fig. 1
- Fig. 2
- fig 3
- fig.4
- Fig. 1
- Fig. 2
- Fig. 3
- Fig. 4
- Fig.4
- fig1
- fig 2
- fig 3
- fig1
- fig2
- fig3
- Fig 1
- Fig 2
- fig 3
- fig 4
- fig5
- Fig 1
- fig 2
- fig3
- fig 4
- Fig 1
- fig 2
- fig 3
- fig 4
- fig 5
- fig 1
- Fig 2
- FIG1
- FIG2
- fig1
- fig 2
- fig 2
- Fig 1
- fig2
- fig 3
- fig4
- fig5
- fig6
- Fig 1
- fig2
- fig3
- fig4
- fig 5
- fig 6
- Fig.1
- Fig.2
- Fig.3
- Fig.4
- fig 1
- fig 2
- fig1
- fig 2
- Fig 1
- fig 2
- fig3
- fig4
- fig1
- fig2
- fig3
- fig4
- Fig.2
- Fig.1
- Fig.2
- fig1
- fig2
- fig 3
- fig 4
- fig1
- fig2
- fig3
- fig 4
- Pr Jean-François Joanny
- Fig1 - François Amblard
- Fig2.Amblard
- Patricia Bassereau
- logo CNRS
- logo umpc
- u
- .
- equipe.isambert
- equipe.isambert1
- news 1
- coli
- concentration wave of E. coli bacteria in a microchannel
- Amphiphilic Poly(ethylene oxide)-block-poly(butadiene-graft-liquid crystal) Copolymers: Synthesis and Self-Assembly in Water
- Binding, reconstitution and 2D crystallization of membrane or soluble proteins onto functionalised lipid layer observed in situ
- collectively migrating epithelium biophys cover
- Fig 5. Non-conservation of network motifs
- Fig 4. Model of biomolecular networks under duplication-divergence evolution
- Fig 3. Whole genome duplications in evolution
- Fig 2. Novel nanostructures made of DsrA ncRNA of E.coli
- Fig 1. RNA synthetic biology
- Fig 1. RNA synthetic biology
- Fig 1. RNA synthetic biology
- Fig 4. Model of biomolecular networks under duplication-divergence evolution
- Fig 4. Model of biomolecular networks under duplication-divergence evolution
- Fig 5. Expansion of signaling networks by whole genome duplication
- Fig. 1 Motor oscillations
- Fig. 2 Tissue competition
- Fig. 2 Compétition entre tissus
- Fig. 1 Oscillations de moteurs moléculaires
- Expansion of signaling networks by whole genome duplication
- image
- Brigitte da Silva
- Giulia carzedda
- laurence turpin
- Agnes verin
- anne-christine brunet
- Aurelie
- Fahima
- John Manzi
- jean-hugues
- rémy
- benoit
- Brigitte Da Silva
- Brigitte Da Silva
- Giulia Carzedda
- Laurence Turpin
- Agnès Vérin
- Anne-Christine Brunet
- Aurélie Di Cicco
- Fahima Faqir
- Jean-Hugues Codarbox
- Rémy Fert
- Benoît Lemaire
- Giulia Carzedda
- Optical tweezer and edge fluctuation setup
- Functional and structural analysis of BmrC/BmrD abacterial heterodimeric MDR transporter
- Functional and structural analysis of BmrC/BmrD bacterial heterodimeric MDR transporter
- John Manzi
- Structural changes in liquid crystal polymer vesicles induced by temperature variation and magnetic fields
- Biocompatible gold nanorods for cancer research
- Self-assembly of amphiphilic liquid crystal block copolymers containing a cholesteryl mesogen: Effects of block ratio and solven
- Mechanical measurements of biomimetic cortex
- Bleb mechanics
- Confined Actin Gel Mechanics
- cover collective
- collective
- collective1
- collective
- collective
- Smectic polymer micellar aggregates with temperature-controlled morphologies
- Smectic polymer micellar aggregates with temperature-controlled morphologies
- Smectic polymer micellar aggregates with temperature-controlled morphologies
- Smectic polymer micellar aggregates with temperature-controlled morphologies
- C. elegans sperm cell crawling on a substrate.
- Actin filled liposome
- Actin Beads and VASP
- C. elegans sperm cells transfected with fluorescent MSP
- Multilayered liposome observed by cryoEM
- electron microscopy images
- 2D crystals of BmrC/BmrD
- 2D crystals of BmrC/BmrD, a bacterial heterodimeric ABC transporter
- Multilayered liposome obserbed by cryoEM
- 2D crystals of Light Harversting Complex, LH2, from Rba. sphaeroides
- Single particle analysis of 300kDa LH1-RC-PufX core complex of Rbs. veldkampii
- Atomic model of LH1-RC-PuhX from Rba. sphaeroides
- Atomic Model of LH1-RC-PufX from Rba. sphaeroides
- Single particle analysis by cryoElectron Microscopy of a small non symemetrical membrane protein
- 2D crystals of Light Harversting Complex, LH2, from Rba. sphaeroides
- 3D reconstruction of LH1-RC-PufX, 280 Kda, at 12 A resolution
- 3D reconstruction of LH1-RC-PufX, 280 kDa, at 12 A resolution
- 2D crystals of Light Harversting Complex, LH2, from Rba. sphaeroides
- schematic model of the coupling between the non equivalent catalytic sites of ABC transporters
- schematic model of the coupling between the non equivalent catalytic sites of ABC transporters
- schematic model of the coupling between the equivalent catalytic sites of ABC transporters
- Tubular to planar membranes upon conformational changes of BmrA, a ABC transporter
- Orientation of the Shiga toxin B subunit toward the membrane
- Pores in lipid membrane
- Functionalyzed tubes made of cerebroside lipids
- Polymersomes
- Light sensitive Polymersomes
- Tubular to planar membrane transition mediated by transmembrane protein
- Biocompatible gold nanorods for cancer research
- visuel publication
- Liposome with actin cortex aspirated in a micropipet
- Actin filled liposomes form a cortex if polymerization is activated at the membrane
- Cell fragments show spontaneous osscilation
- Symmetry breaking of the actin gel around a bead
- C. elegans embryo during ventral closure
- Figure 2
- Figure2
- TEM of gold nanorod
- Figure 1
- Figure 3
- Map Institut Curie, Circle Meeting 2012
- diffusion rotationnelle
- Karen Brémond
- Karen Brémond
- Speed and orientation in a migrating monolayer.
- stencils
- bact wave
- cells 1
- cell2
- champ vit
- stencils
- MAgnetic Tweezers
- Bassereau-group-2012
- Map_BDD_Amphi_Curie
- TEM images of block copolymer self-assemblies
- simulations modele
- LH1-RC
- LH1-RC Rba. veldkampii
- LH2 from Rba. sphaeroides
- LogoCELTISPHYBIO
- Incorporation of membrane proteins in planar lipid bilayer
- 2D crystallization by the lipid layer
- Reconstitution of membrane proteins in GUVS
Sounds
Videos
- Film 1
- Film 2
- Film 1 - C. Sykes
- Film 2 - C. Sykes
- Film 1 : Oscillations spontanées
- Film 2 - Martin
- Film 1 - Amblard
- Film 1 - Brochard
- Film 2 - Brochard
- Film 3 - Brochard
- Film 4 - Brochard
- S2 RNA regulatory module
- A2 activated S2 RNA regulatory module
- Red Blood Cell Flickering
- E. coli collective migration
- 2D crystallization of proteins onto functionalyzed lipid layer
Partagez
Tubular to planar membranes upon conformational changes of BmrA, a ABC transporter
BmrA, a bacterial homodimeric ABC transporter has been reconstituted in a lipid bilayer at high protein density. BmrA provides a ATP dependant efflux transport of drugs including antibiotics and anticancer agents. BmrA trapped in different catalytic states has been reconstituted in lipid membrane and the result analyzed by electron microscopy. (A) BmrA wild type in absence of ATP. Formation of tubular membranes (B) WT in presence of ATP. Formation of planar membrane. (C) E504Q mutant able to bind but not to hydrolyze ATP. Recosntitution in absence of ATP leads to the formation of tubular membranes. (D) Reconstitution in presence of ATP leads to the formation of planar membrane. Overall, the data are show that binding rather than hydrolysis of ATP is the drivent force of a large conformational change of BmrA, likely related to the drug translocation. From
Orelle, C., Gubellini, F., Durand, A., Marco, S., Lévy, D., Gros, P., Di Pietro, A., and Jault, J. M. (2008) Conformational change induced by ATP binding in the multidrug ATP-binding cassette transporter BmrA, Biochemistry 47, 2404-2412.