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fig2
La compression mécanique des cellules du stomodeum induit l'expression du gène Twist dans des embryons de drosophile.
(A) Dans des embryons de drosophile de stade 6, les cellules du stomodeum (participant à la formation du futur intestin antérieur, flèches rouges) ne sont pas comprimées ; elles sont comprimées au stade 7 par le mouvement de convergence-extension antérieur du tissu ventral et, éventuelle du tissu dorsal (flèches jaunes). (B) Le gène Twist est faiblement exprimé dans les cellules du stomodeum au début du stade 6 (flèches rouges) ; 10 minutes après le début de la compression au stade 7, le gène Twist commence à s'exprimer fortement. (C) Les mutants des gènes bcd, nos et tsl, qui ne subissent pas le mouvement de convergence-extension et par conséquent ne compriment pas les cellules du stomodeum, n'induisent pas de forte expression du gène Twist dans ces cellules. (D) Une forte expression du gène Twist dans les cellules du stomodeum de cellules d'embryons mutants est induite par compression mécanique des cellules en appliquant un pointe de 50 µm.