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C. elegans sperm cells transfected with fluorescent MSP

C. elegans sperm cells transfected with fluorescent MSP

A worm expressing fluorescently-labeled MSP.  Left:  transmitted light image, right: epifluorescence image.  O = oocyte, G= gonad arm, S= spermatheca, and E = fertilized embryo. Fluorescence is only seen in the spermatheca, where sperm cells are stored. b) A representative field of active and unactive fluorescent sperm cells. Left: transmitted light image, right: confocal fluorescence image.  In unactive round sperm cells, fluorescence is fairly uniform (arrows), while in activated cells, fluorescence is concentrated in the lamellipodia and displays a bright patches (arrowheads).  Scale bars = 5 mm.